Glp1r Knockout cell line (H9c2(2-1))

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location： Home > Products > KO Cell Lines > Glp1r Knockout cell line (H9c2(2-1))

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Glp1r Knockout cell line (H9c2(2-1)) Order

Glp1r Knockout cell line (H9c2(2-1))
Catalog#
YKO-R010
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Glp1r Knockout cell line (H9c2(2-1))
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Glp1r Knockout cell line (H9c2(2-1))
Morphology
myoblast,adherent
Passage ratio
1:3~1:4
Species
Rat
Gene Name
Glp1r
Gene ID
25051
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10% FBS
Target region
Knockout region: Exon 2 (based on transcript Glp1r-201)
Validation: PCR+Sanger Sequencing
Glp1r Knockout cell line (H9c2(2-1)) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Glp1r gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H9c2(2-1) cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Glp1r gene knockout Glp1r Knockout cell line (H9c2(2-1)) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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